Review



human aortic adventitial fibroblasts  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Lonza human aortic adventitial fibroblasts
    Human Aortic Adventitial Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/human+aortic+adventitial+fibroblasts/pm37952087-253-0-7
    Average 90 stars, based on 1 article reviews
    human aortic adventitial fibroblasts - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Mechanistic Design of Polymer Nanocarriers to Spatiotemporally Control Gene Silencing.
    Article Snippet: Current siRNA delivery systems lack the ability to precisely tune siRNA release and maximize gene silencing in a spatiotemporal manner.. Herein, we investigate photoresponsive block copolymer solution assemblies, for which stimuli-triggered changes in polymer structure altered nanocarrier stability and defined siRNA activity.. Uniquely, our biomaterials design enabled the development and validation of a simple kinetic model that accurately predicted the extent of intracellular nanocarrier disassembly and silencing.

    Article Title: Casein kinase 2 activity is a host restriction factor for AAV transduction.
    Article Snippet: .. Human aortic adventitial fibroblasts were purchased from Lonza (CC-7014), human cardiac fibroblasts were obtained from SigmaAldrich (306-05A) and cultured according to the manufacturers’ instructions. .. Differentiation to cardiomyocytes was conducted using modified protocol by Lian et al.65 Human iPSCs were seeded on a 24-well plate (30,000 cells per well) and at approximately 90%–100% confluence, stimulated with 12 mM (DMB01 line) or 10 mM (DMB02 line) CHIR99021 (Sigma-Aldrich) in RPMI 1640 medium supplemented with B-27 minus insulin (Gibco) for 24 h (day 0).

    Article Title: Loss of Cardio-Protective Effects at the ADAMTS7 Locus Due to Gene-Smoking Interactions
    Article Snippet: .. 28 Analyses of ADAMTS7 and CHRNB4-A3-A5 gene expression in vascular cells and tissues ADAMTS7 and CHRNB4-A3-A5 gene expression in vascular cells ADAMTS7 and CHRNB4-A3-A5 mRNA levels were measured in cultured human coronary artery smooth muscle cells (HCASMC; Lonza CC-2583, Lonza Walkersville, MD), human coronary artery endothelial cells (HCAEC, Lonza CC-2585), human aortic smooth muscle cells (HAoSMC, Lonza CC-2571), human aortic endothelial cells (HAoEC, Lonza CC-2535), human aortic adventitial fibroblasts (HAoAF, Lonza CC-7014), and human acute monocytic leukemia cell line (THP-1, ATCC TIB-202). ..

    Article Title: Attenuation of Maladaptive Responses in Aortic Adventitial Fibroblasts through Stimuli-Triggered siRNA Release from Lipid-Polymer Nanocomplexes
    Article Snippet: .. [ 21 ] 4.3 Cell culture Human aortic adventitial fibroblasts were obtained from Lonza (Walkersville, MD) and cultured following Lonza’s protocol in stromal cell basal medium (SCBM) supplemented with the stromal cell growth medium (SCGM) SingleQuot Kit. ..

    Gene Expression:

    Article Title: Loss of Cardio-Protective Effects at the ADAMTS7 Locus Due to Gene-Smoking Interactions
    Article Snippet: .. 28 Analyses of ADAMTS7 and CHRNB4-A3-A5 gene expression in vascular cells and tissues ADAMTS7 and CHRNB4-A3-A5 gene expression in vascular cells ADAMTS7 and CHRNB4-A3-A5 mRNA levels were measured in cultured human coronary artery smooth muscle cells (HCASMC; Lonza CC-2583, Lonza Walkersville, MD), human coronary artery endothelial cells (HCAEC, Lonza CC-2585), human aortic smooth muscle cells (HAoSMC, Lonza CC-2571), human aortic endothelial cells (HAoEC, Lonza CC-2535), human aortic adventitial fibroblasts (HAoAF, Lonza CC-7014), and human acute monocytic leukemia cell line (THP-1, ATCC TIB-202). ..

    other:

    Article Title: Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis
    Article Snippet: Isolated LYVE-1 positive cells were cultured in DMEM medium supplemented with 20% FBS, 0.1 mg/mL heparin (Sigma-Aldrich), 0.01 mg/mL endothelial cell growth supplement (Sigma), and 0.05 mg/mL gentamycin (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) on 0.1% gelatin-coated culture plates.



    Similar Products

    93
    PromoCell human aortic adventitia fibroblasts
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Aortic Adventitia Fibroblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/Human+Aortic+Adventitial+Fibroblasts/pmc12212911-56-0-9
    Average 93 stars, based on 1 article reviews
    human aortic adventitia fibroblasts - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    PromoCell human aortic adventitial fibroblasts
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Aortic Adventitial Fibroblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/Human+Aortic+Adventitial+Fibroblasts/10__1016_slash_j__ard__2025__05__477-80-0-12
    Average 93 stars, based on 1 article reviews
    human aortic adventitial fibroblasts - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    PromoCell haoaf
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Haoaf, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/Human+Aortic+Adventitial+Fibroblasts/pmc11512639-163-0-4
    Average 93 stars, based on 1 article reviews
    haoaf - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Lonza human aortic adventitial fibroblasts
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Aortic Adventitial Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/human+aortic+adventitial+fibroblasts/pm37952087-253-0-7
    Average 90 stars, based on 1 article reviews
    human aortic adventitial fibroblasts - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    PromoCell human aortic adventitial fibroblasts haoafs
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Aortic Adventitial Fibroblasts Haoafs, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/Human+Aortic+Adventitial+Fibroblasts/pmc10864968-40-0-5
    Average 93 stars, based on 1 article reviews
    human aortic adventitial fibroblasts haoafs - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    PromoCell human vascular fibroblast cells
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Vascular Fibroblast Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/Human+Aortic+Adventitial+Fibroblasts/pm36547542-94-9-18
    Average 93 stars, based on 1 article reviews
    human vascular fibroblast cells - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Lonza human aortic adventitial fibroblasts (aoaf; de-identified 53 yo male donor; lonza, allendale, nj)
    The expression of <t>fibroblast</t> phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in <t>adventitia,</t> media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)
    Human Aortic Adventitial Fibroblasts (Aoaf; De Identified 53 Yo Male Donor; Lonza, Allendale, Nj), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+adventitial+fibroblasts/human+aortic+adventitial+fibroblasts/pm33231137-110-0-10
    Average 90 stars, based on 1 article reviews
    human aortic adventitial fibroblasts (aoaf; de-identified 53 yo male donor; lonza, allendale, nj) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    The expression of fibroblast phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in adventitia, media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)

    Journal: Rheumatology (Oxford, England)

    Article Title: Distinct landscapes of fibroblast subtypes in arteries of patients with giant cell arteritis

    doi: 10.1093/rheumatology/keaf143

    Figure Lengend Snippet: The expression of fibroblast phenotypic markers in temporal artery biopsies (TAB). ( A ) Representative picture of the immunohistochemical (IHC) staining of CD90, fibroblast activation protein α (FAP), podoplanin (PDPN), CD248 and α-smooth muscle actin (α-SMA) in giant cell arteritis (GCA)-affected TAB. ( B ) IHC staining in control TAB. ( C ) Scoring of fibroblast markers in adventitia, media, and intima (percentage of positive cells per layer). NS, not significant; * P < 0.05, ** P < 0.01. ( D ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected TAB. PDPN, red; CD90, green; FAP, yellow; CD248, red; α-SMA, yellow; colocalization of CD90 and PDPN is shown in yellow, colocalization of CD90 and FAP is shown in magenta, colocalization of CD90 and αSMA is shown in magenta, colocalization of CD90 and CD248 is shown in cyan. Arrows indicate double positive areas. A, adventitia; M, media; I, intima. For GCA-TAB, intima includes media–intima (MI) and inner intima (II)

    Article Snippet: Human aortic adventitia fibroblasts (HAoAF, C-12380) were obtained from PromoCell, Heidelberg, Germany.

    Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Activation Assay, Control, Immunofluorescence, Staining

    Fibroblast distribution patterns in aorta tissues. ( A ) Expression of fibroblast markers (CD90, fibroblast activation protein [FAP], podoplanin [PDPN], CD248, α-smooth muscle actin [α-SMA]) and cytokines related to remodelling (TGF-β, fibroblast growth factor 21 [FGF21], platelet-derived growth factor B [PDGFB]) in GCA-affected aorta tissues. Adv, adventitia; Med, media; Int, intima. ( B ) Scoring of fibroblast markers and cytokines related to remodelling in adventitia and media. NS, not significant; * P < 0.05, ** P < 0.01. ( C ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected aorta tissues (structurally disrupted media). CD90, green; PDPN, red; CD24, red; FAP, yellow; α-SMA, yellow. Colocalization of CD90+CD248, CD90+PDPN is shown in cyan; colocalization of CD90+FAP, CD90+α-SMA is shown in magenta. Arrows indicate double positive areas. ( D ) Masson trichrome staining and collagen scoring in aorta tissues. NS: not significant; * P < 0.05

    Journal: Rheumatology (Oxford, England)

    Article Title: Distinct landscapes of fibroblast subtypes in arteries of patients with giant cell arteritis

    doi: 10.1093/rheumatology/keaf143

    Figure Lengend Snippet: Fibroblast distribution patterns in aorta tissues. ( A ) Expression of fibroblast markers (CD90, fibroblast activation protein [FAP], podoplanin [PDPN], CD248, α-smooth muscle actin [α-SMA]) and cytokines related to remodelling (TGF-β, fibroblast growth factor 21 [FGF21], platelet-derived growth factor B [PDGFB]) in GCA-affected aorta tissues. Adv, adventitia; Med, media; Int, intima. ( B ) Scoring of fibroblast markers and cytokines related to remodelling in adventitia and media. NS, not significant; * P < 0.05, ** P < 0.01. ( C ) Triple immunofluorescence staining of CD90/FAP/PDPN and CD90/α-SMA/CD248 in GCA-affected aorta tissues (structurally disrupted media). CD90, green; PDPN, red; CD24, red; FAP, yellow; α-SMA, yellow. Colocalization of CD90+CD248, CD90+PDPN is shown in cyan; colocalization of CD90+FAP, CD90+α-SMA is shown in magenta. Arrows indicate double positive areas. ( D ) Masson trichrome staining and collagen scoring in aorta tissues. NS: not significant; * P < 0.05

    Article Snippet: Human aortic adventitia fibroblasts (HAoAF, C-12380) were obtained from PromoCell, Heidelberg, Germany.

    Techniques: Expressing, Activation Assay, Derivative Assay, Immunofluorescence, Staining

    The relationship between fibroblast activating protein (FAP) and human aortic fibroblast proliferation. ( A , B ) Triple immunofluorescent staining of CD90/FAP/Ki67 in GCA-affected temporal artery ( A ) and aorta (structurally disrupted media) ( B ). Ki67, red; CD90, green; FAP, yellow; colocalization of CD90/FAP, cyan; arrows indicate CD90 + FAP + Ki67 + cells. A, adventitia; M, media; I, intima; GCA, giant cell arteritis. ( C ) TGF-β1 5 ng/ml 48 h promoted mRNA expression of FAP and proliferation in human aortic adventitial fibroblasts (HAoAF). Proliferation rate was calculated as Ki67 positive nuclei divided by 4′,6-diamidino-2-phenylindole positive nuclei; Ki67, red. ( D ) HAoAF were transfected with small interfering RNA (siRNA) targeting FAP, and were treated with TGF-β1 6 h later. After 24 h, cells were collected for qPCR; 72 h later, cells were collected for Ki67 staining. FAP knockdown decreased the effect of TGF-β1 on cell proliferation. FAP, red; Ki67, green. NB si: non-binding small interfering RNA; FAP siRNA: small interfering RNA targeting FAP. NS: not significant; * P < 0.05, ** P < 0.01

    Journal: Rheumatology (Oxford, England)

    Article Title: Distinct landscapes of fibroblast subtypes in arteries of patients with giant cell arteritis

    doi: 10.1093/rheumatology/keaf143

    Figure Lengend Snippet: The relationship between fibroblast activating protein (FAP) and human aortic fibroblast proliferation. ( A , B ) Triple immunofluorescent staining of CD90/FAP/Ki67 in GCA-affected temporal artery ( A ) and aorta (structurally disrupted media) ( B ). Ki67, red; CD90, green; FAP, yellow; colocalization of CD90/FAP, cyan; arrows indicate CD90 + FAP + Ki67 + cells. A, adventitia; M, media; I, intima; GCA, giant cell arteritis. ( C ) TGF-β1 5 ng/ml 48 h promoted mRNA expression of FAP and proliferation in human aortic adventitial fibroblasts (HAoAF). Proliferation rate was calculated as Ki67 positive nuclei divided by 4′,6-diamidino-2-phenylindole positive nuclei; Ki67, red. ( D ) HAoAF were transfected with small interfering RNA (siRNA) targeting FAP, and were treated with TGF-β1 6 h later. After 24 h, cells were collected for qPCR; 72 h later, cells were collected for Ki67 staining. FAP knockdown decreased the effect of TGF-β1 on cell proliferation. FAP, red; Ki67, green. NB si: non-binding small interfering RNA; FAP siRNA: small interfering RNA targeting FAP. NS: not significant; * P < 0.05, ** P < 0.01

    Article Snippet: Human aortic adventitia fibroblasts (HAoAF, C-12380) were obtained from PromoCell, Heidelberg, Germany.

    Techniques: Staining, Expressing, Transfection, Small Interfering RNA, Knockdown, Binding Assay